.
The Open Protein Structure Annotation Network
PDB Keyword
.

3pl0

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of a bsmA homolog (Mpe_A2762) from Methylobium petroleophilum PM1 at 1.91 A resolution. To be published
    Site JCSG
    PDB Id 3pl0 Target Id
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Molecular Characteristics
    Source
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Alias Ids
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    TPS26547,YP_001021951.1, 325753
    Molecular Weight
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Da.
    Residues
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Isoelectric Point
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Sequence
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
      BLAST   FFAS

    Structure Determination
    Method XRAY
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Chains 2
    Resolution (Å)
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Rfree
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Matthews' coefficent 2.49 Rfactor 0.1694
    Waters
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Solvent Content 50.50

    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)

    Ligand Information
    Ligands
    Metals

    Jmol

     
    parameter 'target' could not convert from 'list' to 'str' (click for details)
    Google Scholar output for 3pl0

    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)

    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)

    Protein Summary

    This protein is a homolog of BsmA, which is involved in quorum sensing-controlled biofilm development in
    Serratia liquefaciens MG1.The exact function is unknown. This protein could be an enzyme or substrate-binding protein. A large, well-defined, conserved cavity is located on the protein surface (Fig2). 

     

    This is the first structure of family DUF2257. The structure reveals that this family are novel dioxygenases. Active site residues for this protein: His165, Asp167, His218. Based on similarity to other proteins, this protein would bind Fe2+ and 2OG (2-oxoglutarate). As a result, this family can be assigned a member of  2OG-FeII_Oxy (PF03171), even though sequence similarity is only restricted to catalytic residues.

     

    Potential residues for substrate specificity: Arg65 (c=conserved), Arg104 (c), Arg67 (c), His89,Gln91 (c), Phe106 (c), His146 (c), Phe148 (hydrophobic), Val241 (hydrophobic), Thr243.

     

     Fig. 1. monomer

    fr14672c.png

     

    Fig. 2. surface

    fr14672c-mon.png

    From Pfam:

    This sequence matches family DUF2257 (PF10014), for which there are no other structures.

    From Krishna:

    This protein shares remote sequence similarity with the
    Double-stranded beta-helix fold of SCOP and should be classified as
    such ( http://scop.mrc-lmb.cam.ac.uk/scop/data/scop.b.c.bdi.A.html ).
    In addition, there is also reasonable similarity with DUF2086
    (PF09859) and the CsiD family of non-heme Fe(II)-dependent
    oxygenase(PF08943). Therefore both DUF2086 and DUF2257 should be
    included as members of the Cupin clan of Pfam.
    

    Ligand Summary

    Reviews

    References

     

    No references found.

    Tag page
    • No tags

    Files (2)

    FileSizeDateAttached by 
     fr14672c-mon.png
    surface of fr14672c
    210.87 kB19:37, 22 Sep 2010qxuActions
     fr14672c.png
    monomer
    137.78 kB19:35, 22 Sep 2010qxuActions
    You must login to post a comment.
    All content on this site is licensed under a Creative Commons Attribution 3.0 License
    Powered by MindTouch