The Open Protein Structure Annotation Network
PDB Keyword


    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of a formyltetrahydrofolate deformylase (purU, PP_1943) from PSEUDOMONAS PUTIDA KT2440 at 2.05 A resolution. To be published
    Site JCSG
    PDB Id 3nrb Target Id 398622
    Molecular Characteristics
    Source Pseudomonas putida kt2440
    Alias Ids TPS29654,NP_744095.1, _0004.004077_, 332387 Molecular Weight 32059.84 Da.
    Residues 286 Isoelectric Point 6.00
    Sequence mnknnqyvlslacqdapgivsevstflfnnganiveaeqfndedsskffmrvsveipvagvndfnsafg kvvekynaewwfrprtdrkkvvimvskfdhclgdllyrhrlgeldmevvgiisnhprealsvslvgdip fhylpvtpatkaaqesqiknivtqsqadlivlarymqilsddlsaflsgrcinihhsflpgfkgakpyh qahtrgvkligatahfvtadldegpiiaqdvehvshrdsaedlvrkgrdierrvlsravllfledrliv ngertvvfad
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 4
    Resolution (Å) 2.05 Rfree 0.240
    Matthews' coefficent 2.43 Rfactor 0.184
    Waters 785 Solvent Content 49.38

    Ligand Information


    Google Scholar output for 3nrb
    1. Crystal structure of tandem ACT domain_containing protein ACTP from Galdieria sulphuraria
    E Bitto, DJ Kim, CA Bingman, HJ Kim - Proteins: Structure, , 2012 - Wiley Online Library

    Protein Summary

    Pfam: this target matchs both  ACT and Formyl_trans_N families in Pfam.

    Gene NP_744095 from Pseudomonas putida kt2440 encodes a protein with 286 residues.  In the each asymmetric unit cell, four subunits forms a tetramer as the biolomolecule suggested by PISA.  Analytical size exclusion chromatography also indicates a tetramer is a significantoligomerization state.  Sequence alignment for NCI Blast indicates that the N-terminal domain belongs to the  N-terminal ACT domain of formyltetrahydrofolate deformylase and the C-terminal domain belongs to the Formyl_trans_N super-family.   Dali and SSM also suggests this protein be a structural homolog to Phosphoribosylglycinamide formyltransferase and GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE, respectively.




    Fig. 1 The monomer of protein NP_744095 with two domains.




    Fig. 2  The teramer of protein NP_744095 as the biomolecule.











    Ligand Summary




    No references found.

    Tag page
    • No tags

    Files (2)

    FileSizeDateAttached by 
    No description
    502.38 kB23:43, 21 Jun 2010kevinjinActions
    No description
    146.83 kB23:43, 21 Jun 2010kevinjinActions
    You must login to post a comment.
    All content on this site is licensed under a Creative Commons Attribution 3.0 License
    Powered by MindTouch