The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of an ABC-type branched-chain amino acid transporter (RPA4397) from Rhodopseudomonas palustris CGA009 at 1.50 A resolution. To be published
    Site JCSG
    PDB Id 3n0x Target Id 405167
    Molecular Characteristics
    Source Rhodopseudomonas palustris cga009
    Alias Ids TPS33721,NP_949733.1, BIG_798, 322696 Molecular Weight 40611.28 Da.
    Residues 373 Isoelectric Point 5.85
    Sequence addlkialiygktgpleayakqtetglmmgleyatkgtmtldgrkivvitkddqskpdlskaalaeayq ddgadiaigtsssaaaladlpvaeenkkilivepavadqitgekwnryifrtgrnssqdaisnavaigk qgvtiatlaqdyafgrdgvaafkealaktgatlateeyvpttttdftavgqrlfdalkdkpgkkiiwvi wagggdpltklqdmdpkrygielstggnilpalaaykrlpgmegatyyyydipknpinewlvtehqkrf nappdfftaggfsaamavvtavqkakstdtekliaamegmefdtpkgkmvfrkedhqalqsmyhfkvkv dpavawavlepvrelkieemnipiknkk
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 1.50 Rfree 0.172
    Matthews' coefficent 2.12 Rfactor 0.148
    Waters 539 Solvent Content 41.90

    Ligand Information


    Google Scholar output for 3n0x
    1. Blind prediction of quaternary structures of homo-oligomeric proteins from amino acid sequences based on templates
    M Morita, M Kakuta, K Shimizu, S Nakamura - 2012 - hoajonline.com

    Protein Summary

    Pfam update: This protein is in our ANF_receptor (PF01094) family, for which there are many other structures. It is probably Q6N1K8.

    It is present as a monomer in the crystal structure. The putative active site is at Arg138 and the structure has a glycerol molecule from the cryoprotectant modeled at this site:


    Related proteins with similar structure can be found through the FFAS link above. A search with EBI-SSM of other proteins of similar structure results in significant top hits (Q-scores > 0.38 and rmsds < 2.9A) to PDB ids 3hut (branched-chain amino acid ABC transporter), 2lbp (leucine-binding protein), 2liv (leucine-binding protein), 3h5l (branched-chain amino acid aBC transporter), 3i45 (putative twin-arginine translocation pathway signal protein) and 1z15 (leu/isoleucine/valine-bindingp protein).

    Ligand Summary




    No references found.

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