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The Open Protein Structure Annotation Network
PDB Keyword
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3mwx

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of a putative galactose mutarotase (BSU18360) from BACILLUS SUBTILIS at 1.45 A resolution. To be published
    Site JCSG
    PDB Id 3mwx Target Id
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    Molecular Characteristics
    Source
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    Alias Ids
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    TPS30551,NP_389718.1, _0116.000991_, _0003.003178_, 327835
    Molecular Weight
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    Da.
    Residues
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    Isoelectric Point
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    Sequence
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      BLAST   FFAS

    Structure Determination
    Method XRAY
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    Chains 2
    Resolution (Å)
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    Rfree
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    Matthews' coefficent 2.73 Rfactor 0.148
    Waters
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    Solvent Content 54.96

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    Ligand Information
    Ligands
    Metals

    Jmol

     
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    Google Scholar output for 3mwx
    1. Assessment of ligand_binding residue predictions in CASP9
    T Schmidt, J Haas, TG Cassarino - Structure, Function, and , 2011 - Wiley Online Library
     
    2. Blind prediction of quaternary structures of homo-oligomeric proteins from amino acid sequences based on templates
    M Morita, M Kakuta, K Shimizu, S Nakamura - 2012 - hoajonline.com
     

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    Protein Summary

    The protein YoxA (renamed as GalM [Ref]) is an aldose-1-epimerase/Galactose mutarotase, supported by both sequence analysis and biochemical characterization (reference 1).

    The all-beta structure is most similar to Slr1438 (

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    , rmsd 1.76A for 270 Ca atoms, seq id 20%), and
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    , both by NESG. It is also similar to other characterized GalMs such as pdbs
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    /
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    [Ref], which allows identification of the active site
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    near residues
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    ,  
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    ,  
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    ,  
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    etc (occupied by a glycerol, Fig 2).

    This structure is homologous (30% seq id) to 397745. 397745 shares 30% seq id to BsGalM, and is highly similar in structure  to BsGalM (rmsd 1.3A for 309 Ca).

     

    Fig. 1 Structure of 397864

    MI15933A-mono.png

    Fig. 2. Active site of BsGalM

    mi15933b-act.png

    Fig 3. Structure of 397745 in complex with PEG and Tartrate

    mi15933a.png

    Ligand Summary

    Reviews

    References

     

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