The Open Protein Structure Annotation Network
PDB Keyword


    Title Crystal structure of Calcium/calmodulin-dependent kinase II association domain (YP_001814158.1) from EXIGUOBACTERIUM SP. 255-15 at 2.59 A resolution. To be published
    Site JCSG
    PDB Id 3ksp Target Id 390842
    Molecular Characteristics
    Source Exiguobacterium sibiricum 255-15
    Alias Ids TPS18290,YP_001814158.1, 3.10.450.50, 85527 Molecular Weight 15095.04 Da.
    Residues 128 Isoelectric Point 4.94
    Sequence mepsakhlqlqtllserhaylmegnreamhqllssdfsfidgqgrqfdaetyldhyvdpdqiqwsnqis esmvvevfettalvqeivedhfsygrsmyigrfrsvslyhwanegwkwhfhqltpldps
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 2.59 Rfree 0.237
    Matthews' coefficent 3.92 Rfactor 0.201
    Waters 8 Solvent Content 68.66

    Ligand Information


    Google Scholar output for 3ksp

    Protein Summary

    Gene Exig_1688 from Exiguobacterium sp. 255-15 encodes the protein YP_001814158. It is related to a second SnoaL-like family of Pfam (not publicly available yet) which is also part of the NTF2 clan.

    SCOP classifies 3ksp in the NTF-2 like superfamily, ketosteroid isomerase like family. The 3ksp monomer structure resembles a hotdog fold, with a helix lying in the beta-sheet.





    The most likely oligomeric state of the protein could be a dimer, shown below in two orthogonal views.

    MG14479A_dim1.png  MG14479A_dim2.png


    A buffer molecules, CHES, is bound to the protein and could mimic a substrate, as shown below.


    Ches molecule (NHE) is shown in stick representation in yellow whicl the interacting residues are shown in green.


    There are several structures similar to 3ksp, listed below.

    DALI Structurally Similar Proteins
    N PDB Z-score RMSD LALI NRES %ID TITLE (JCSG structures highlighted in red)
    1 3fsd 18.1 1.6 118 121 17 NTF2-LIKE PROTEIN OF UNKNOWN FUNCTION IN
    2 2r4i 17.9 1.9 121 121 16 UNCHARACTERIZED PROTEIN
    3 3cnx 15.7 2.3 123 138 15 UNCHARACTERIZED PROTEIN
    4 3hx8 15.0 2.5 121 128 7 PUTATIVE KETOSTEROID ISOMERASE
    5 3ef8 14.8 2.7 127 149 13 PUTATIVE SCYALONE DEHYDRATASE
    6 3gzr 14.7 2.8 125 142 13 UNCHARACTERIZED PROTEIN WITH A NTF2-LIKE FOLD
    7 3hzp 14.6 2.4 121 128 15 NTF2-LIKE PROTEIN OF UNKNOWN FUNCTION
    8 3b8l 14.6 2.3 123 143 13 UNCHARACTERIZED PROTEIN
    9 1tp6 14.6 2.2 113 126 12 HYPOTHETICAL PROTEIN PA1314
    10 3fka 14.4 1.9 112 119 12 UNCHARACTERIZED NTF-2 LIKE PROTEIN



    A superposition of these structures are shown below.


    Color scheme: 1tp6 (green), 2r4i (cyan), 3b8l (lightmagenta), 3cnx (yellow), 3ef8 (salmon), 3fka (lightgrey), 3fsd (slate), 3gzr (orange), 3hx8 (lime), 3hzp (deepteal),  3ksp (hotpink),


    Note from PFAM:

    This is a related to our SnoaL family. We have recently added a second SnoaL-like family to Pfam (not publicly available yet) and this sequence is matching this family. It is also part of our NTF2 clan.

    Ligand Summary




    No references found.

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