The Open Protein Structure Annotation Network
PDB Keyword


    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title The Structure of Mlc Titration Factor A (MtfA/YeeI) Reveals a Prototypical Zinc Metallopeptidase Related to Anthrax Lethal Factor. J.Bacteriol. 194 2987-2999 2012
    Site JCSG
    PDB Id 3khi Target Id 388889
    Related PDB Ids 3dl1 
    Molecular Characteristics
    Source Klebsiella pneumoniae subsp. pneumoniae mgh 78578
    Alias Ids TPS7568,YP_001336084.1, PF06167, 104687 Molecular Weight 30302.69 Da.
    Residues 266 Isoelectric Point 4.77
    Sequence mmfkwpwkaddesgnaempweqalaipvlahlssteqhkltqmaarflqqkrlvalqgleltplhqari amlfclpvlelgiewldgfhevliypapfivddeweddiglvhnqrvvqsgqswqqgpvvlnwldiqds fdasgfnlvvhevahkldtrngdrasgvpliplrevagwehdlhaamnniqdeidlvgesaasidayaa tdpaecfavlseyffsapelfaprfpalwqrfchfyrqdplarrrenglqdegdrrivh
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 1.95 Rfree 0.213
    Matthews' coefficent 2.60 Rfactor 0.183
    Waters 124 Solvent Content 52.76

    Ligand Information


    Google Scholar output for 3khi
    1. The Structure of Mlc Titration Factor A (MtfA/YeeI) Reveals a Prototypical Zinc Metallopeptidase Related to Anthrax Lethal Factor
    Q Xu, AK Ghler, A Kosfeld, D Carlton - Journal of , 2012 - Am Soc Microbiol

    Protein Summary

    YP_001336084.1 from Klebsiella pneumoniae subsp. pneumoniae MGH 78578 has sequence similarity to PF06167 (DUF980) (E-value: 2.6e-129), which overlaps with COG3228: Uncharacterized protein conserved in bacteria [Function unknown][1] (E-value: 4e-96). It is highly similar to MtfA (PeeI, YeeI) of E. coli (sequence idenitity of 76%) which is involved in the regulation of phosphoenolpyruvate:glucose phosphotransferase (ptsG) expression by binding and inactivating the repressor Mlc (MtfA interacts with Mlc with high affinity). As a result, this protein is an MtfA homolog.


    According to Dali, it is structually similar to anthrax lethal factor (ALF) with Z-scores above 8.0 for PDB codes 1j7n and 1yqy: domain 1 (27:263) (rmsd 3.3 for 137 aligned Ca, 10% seq id); domain 3 (550:773) (rmsd 3.3 for 118 aligned Ca, 13% seq id). HHpred and FATCAT top hit is with 1j7n (P-val=9.4e-7; 2.6e-5, respectively). Interestingly, YP_001336084.1 also contains a Zn metal binding site like domain 4 of anthrax, consisting residue His112, His149, His153, Glu212 (nearby Glu150, Glu219 not conserved in ALF). However, there is no metal observed in the structure (Zn?). YP_001336084.1 model does not contain the loop 98-124 which is likely involved in recruiting and binding substrates (probably only ordered in substrate bound form?). We propose that YP_001336084.1 is a metal dependent hydrolase.


    Fig 1: YP_001336084.1 has a fold that is similar to the catalytic domain of ALF


    Ligand Summary





    No references found.

    Tag page

    Files (1)

    FileSizeDateAttached by 
    979.6 kB20:07, 26 Oct 2009qxuActions
    You must login to post a comment.
    All content on this site is licensed under a Creative Commons Attribution 3.0 License
    Powered by MindTouch