.
The Open Protein Structure Annotation Network
PDB Keyword
.

3hzp

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of NTF2-like protein of unknown function MN2A_0505 from Prochlorococcus marinus (YP_291699.1) from Prochlorococcus sp. NATL2A at 1.40 A resolution. To be published
    Site JCSG
    PDB Id 3hzp Target Id
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Molecular Characteristics
    Source
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Alias Ids
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    TPS25733,YP_291699.1, 3.10.450.50, 87417
    Molecular Weight
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Da.
    Residues
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Isoelectric Point
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Sequence
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
      BLAST   FFAS

    Structure Determination
    Method XRAY
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Chains 1
    Resolution (Å)
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Rfree
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Matthews' coefficent 2.08 Rfactor 0.167
    Waters
    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)
    Solvent Content 40.84

    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)

    Ligand Information
    Ligands
    Metals

    Jmol

     
    parameter 'target' could not convert from 'list' to 'str' (click for details)
    Google Scholar output for 3hzp

    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)

    reference to undefined name 'note' Exception of type 'MindTouch.Deki.Script.Runtime.DekiScriptUndefinedNameException' was thrown. (click for details)

    Protein Summary

    Pfam note: I have had to admit to building a DUF for this, as I could find on proper info from anywhere on it. DUF3804 PF12707.

     

    Gene MG14622A encodes the YP_291699.1 protein from Prochlorococcus sp. NATL2A, which is a unicellular cyanobacterium that dominates the temperate and tropical oceans.  The search results from NCBI sequence alignment can not suggest any putative conserved domain. Its sequence belongs to the Ca/calmodulin dependent protein kinase II associaction group (PF08332).

     

    SCOP classifies 3hzp in the alpha+beta class, NTF2-like superfamily, CAMK2A family. Dali search indicates 3hzp carries a NTF2-like fold with a hydrophobic cavity similar to 1JB2, 2R4I, 3FSD and 2UX0.  In this hydrophobic cavity, Arg 118  provides the H-bonding force to hold a  PEG molecule from the crystallization buffer. The interface interaction suggests that the biomolecule of 3hzp is a dimer.

     

     

    391203_2.png

    Figure 1. Structure 3hzp carries an α/β-barrel fold similar to the NTF-2 domain. 

     

    391203_3.png


    Figure 2. The biomolecule of 3hzp should be a dimer based on analysis of its asu.

     

    391203_1.png

    Figure 3.  3HZP (green) is structurally similar to 1JB2, 2R4I, 2UX0 and 3FSD.

     

    391203_4.png

    Figure 4.   Arg 118 in the hydrophobic cavity of  3hzp holds a PEG molecule from the crystallization buffer.
     
     

     

     

     

     

     

     

    References

     

    1.    Chaillan-Huntington, C.,  Butler, P.J.,  Huntington, J.A.,  Akin, D.,  Feldherr, C.,  Stewart, M.   (2001) NTF2 monomer-dimer equilibrium.  J.Mol.Biol.   314: 465-477  

     

     

     

     

     

     

     

     

     

     

     

    Ligand Summary

    Reviews

    References

     

    No references found.

    Tag page
    • No tags
    You must login to post a comment.
    All content on this site is licensed under a Creative Commons Attribution 3.0 License
    Powered by MindTouch