The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of NTF2-like protein of unknown function MN2A_0505 from Prochlorococcus marinus (YP_291699.1) from Prochlorococcus sp. NATL2A at 1.40 A resolution. To be published
    Site JCSG
    PDB Id 3hzp Target Id 391203
    Molecular Characteristics
    Source Prochlorococcus marinus str. natl2a
    Alias Ids TPS25733,YP_291699.1, 3.10.450.50, 87417 Molecular Weight 14573.73 Da.
    Residues 130 Isoelectric Point 4.89
    Sequence msskeeilsileafastermgsffldnatadflfirpsgnpldakgfenmwssgdlvlesaeitkvhkf ellgsnaaicvftlgskftykgtqnddlptvtsifkkidekwkvawmqrssgqsdmtlwne
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 1.40 Rfree 0.184
    Matthews' coefficent 2.08 Rfactor 0.167
    Waters 101 Solvent Content 40.84

    Ligand Information


    Google Scholar output for 3hzp

    Protein Summary

    Pfam note: I have had to admit to building a DUF for this, as I could find on proper info from anywhere on it. DUF3804 PF12707.


    Gene MG14622A encodes the YP_291699.1 protein from Prochlorococcus sp. NATL2A, which is a unicellular cyanobacterium that dominates the temperate and tropical oceans.  The search results from NCBI sequence alignment can not suggest any putative conserved domain. Its sequence belongs to the Ca/calmodulin dependent protein kinase II associaction group (PF08332).


    SCOP classifies 3hzp in the alpha+beta class, NTF2-like superfamily, CAMK2A family. Dali search indicates 3hzp carries a NTF2-like fold with a hydrophobic cavity similar to 1JB2, 2R4I, 3FSD and 2UX0.  In this hydrophobic cavity, Arg 118  provides the H-bonding force to hold a  PEG molecule from the crystallization buffer. The interface interaction suggests that the biomolecule of 3hzp is a dimer.




    Figure 1. Structure 3hzp carries an α/β-barrel fold similar to the NTF-2 domain. 



    Figure 2. The biomolecule of 3hzp should be a dimer based on analysis of its asu.



    Figure 3.  3HZP (green) is structurally similar to 1JB2, 2R4I, 2UX0 and 3FSD.



    Figure 4.   Arg 118 in the hydrophobic cavity of  3hzp holds a PEG molecule from the crystallization buffer.









    1.    Chaillan-Huntington, C.,  Butler, P.J.,  Huntington, J.A.,  Akin, D.,  Feldherr, C.,  Stewart, M.   (2001) NTF2 monomer-dimer equilibrium.  J.Mol.Biol.   314: 465-477  












    Ligand Summary




    No references found.

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