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The Open Protein Structure Annotation Network
PDB Keyword
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3h2d

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of CheC-like superfamily protein (NP_719446.1) from Shewanella oneidensis at 1.86 A resolution. To be published
    Site JCSG
    PDB Id 3h2d Target Id
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    Molecular Characteristics
    Source
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    Alias Ids
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    TPS25288,NP_719446.1, _0037.001839_, 326863
    Molecular Weight
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    Da.
    Residues
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    Isoelectric Point
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    Sequence
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      BLAST   FFAS

    Structure Determination
    Method XRAY
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    Chains 2
    Resolution (Å)
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    Rfree
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    Matthews' coefficent 2.25 Rfactor 0.177
    Waters
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    Solvent Content 45.45

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    Ligand Information
    Ligands
    Metals

    Jmol

     
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    Google Scholar output for 3h2d

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    Protein Summary

    Gene SO_3915 from SHEWANELLA ONEIDENSIS MR-1 encodes the NP_719446 protein which belongs to the CheC-like family (weak e-val=0.0002) PF04509;  and Interpro entry IPR007597. The region represented in this entry is found in CheC, CheX, CheA and FliY proteins.

    SCOP classifies 3h2d in the alpha+beta class, CheC-like (super)family. DALI provides top hits with other CheX proteins like 3hm4 (Z-scr=27), 3h4y (Z-scr=23), 3iic (Z-scr=23), 1xko (Z-scr=20), 1squ (Z-scr=20).

    The 3h2d structure contains a dimer in the asymmetric unit and the crystal packing analysis by PISA suggests that a dimer could be the stable oligomeric form in solution. The biological dimer is shown in Fig 1.

    dimer_MH11753G.png

    Fig 1. Biological dimer of 3h2d.

     

     super_monomer.png

    Fig 2. Superposition of 3h2d monomer (green) with that of CheX 1squ (light blue, rmsd=2.3A, 149 Ca aligned, 21% seq id, Z=20) and CheC 1xkr (magenta, rmsd=3.5A, 139 Ca aligned, 15% seq id, Z=10).

     

    Superposition of the putative active site of 3h2d with those of CheX 1squ and CheC 1xkr shows that the residues are well conserved except for Thr115.

     

     super_active.png

    Figure 3. From top, Glu91, Asn94, Pro116,Thr115 of 3h2d (green), Glu91, Asn94, Pro116, Pro115 of 1squ (light blue) and Glu112, Asn115, Pro138, Pro137 of 1xkr (magenta).

     

    References

    Park, S.Y.,  Chao, X.,  Gonzalez-Bonet, G.,  Beel, B.D.,  Bilwes, A.M.,  Crane, B.R. (2004) Structure and Function of an Unusual Family of Protein Phosphatases; The Bacterial Chemotaxis Proteins CheC and CheX. Mol.Cell 16: 563-574
    Abstract ]    View PubMed Abstract at NCBI

     

    Chao, X.,  Muff, T.J.,  Park, S.Y.,  Zhang, S.,  Pollard, A.M.,  Ordal, G.W.,  Bilwes, A.M.,  Crane, B.R. (2006) A receptor-modifying deamidase in complex with a signaling phosphatase reveals reciprocal regulation. Cell 124: 561-571
    Abstract ]    View PubMed Abstract at NCBI

    Ligand Summary

    Reviews

    References

     

    No references found.

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