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3duk

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of NTF2-like protein of unknown function (YP_544675.1) from METHYLOBACILLUS FLAGELLATUS KT at 2.200 A resolution. To be Published
    Site JCSG
    PDB Id 3duk Target Id
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    Molecular Characteristics
    Source
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    Alias Ids
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    TPS14535,YP_544675.1, 3.10.450.50, 335793
    Molecular Weight
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    Da.
    Residues
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    Isoelectric Point
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    Sequence
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      BLAST   FFAS

    Structure Determination
    Method XRAY
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    Chains 6
    Resolution (Å)
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    Rfree
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    Matthews' coefficent 2.39 Rfactor 0.1629
    Waters
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    Solvent Content 48.61

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    Ligand Information
    Ligands
    Metals

    Jmol

     
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    Google Scholar output for 3duk
    1. Distributed structure determination at the JCSG
    H van den Bedem, G Wolf, Q Xu - Section D: Biological , 2011 - scripts.iucr.org
     

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    Protein Summary

    The Mfla_0564 gene from Methylobacillus flagellatus kt encodes the YP_544675 protein, a nuclear transport factor 2 (NTF2) domain (PF02136). HHPred gives strong hits with the association domain of calcium/calmodulin dependent protein kinase II (PF08332, P-value 3.8E-12 over residues 9-117) and scytalone dehydratase (PF02982, EC 4.2.1.94, P-value 6.2E-11 over residues 4-116). PSI-BLAST provides a hit with the C-terminal region of dehydrogenase ZP_05519550 (e-val=9e-6; seq. id. 32%; gaps 3%).

    According to pre-SCOP, the 3duk structure adopts a cystatin-like fold inside the NTF2-like superfamily, ketosteroid isomerase-like family. DALI top hits are with NTF2-like proteins 3blz (Z=20), 3fka (Z=17) and the ketosteroid isomerase-like protein 3d9r (Z=14).

     

    To do: compare presence/absence of catalytic residues to determine whether this protein could be a scytalone dehydratase.

    Ligand Summary

    Reviews

    References

     

    No references found.

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