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3ddd

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary

    Title Crystal structure of A Putative Acetyltransferase (NP_142035.1) from PYROCOCCUS HORIKOSHII at 2.25 A resolution. To be published
    Site JCSG
    PDB Id 3ddd Target Id
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    Molecular Characteristics
    Source
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    Alias Ids
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    TPS7216,NP_142035.1, 103975
    Molecular Weight
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    Da.
    Residues
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    Isoelectric Point
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    Sequence
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      BLAST   FFAS

    Structure Determination
    Method XRAY
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    Chains 1
    Resolution (Å)
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    Rfree
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    Matthews' coefficent 3.40 Rfactor 0.168
    Waters
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    Solvent Content 63.81

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    Ligand Information
    Ligands
    Metals

    Jmol

     
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    Google Scholar output for 3ddd
    1. Antigen binding proteins to proprotein convertase subtilisin kexin type 9 (PCSK9)
    SM Jackson, B Shan, W Shen, CT King - US Patent 8,030,457, 2011 - Google Patents
     

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    Protein Summary

    379751 shares sequence similarity with the Acetyltransferase (GNAT) family from Pfam (E-value: 1.1e-13). It is likely a monomer, as suggested by crystal packing and size exclusion chromatography. 379751 contains one CoA monomer shown as stick in the presumable active site. 

    379751 also has sequence similarity to a putative acetyltransferase of GNAT family from Shigella flexneri (PDB code: 2pdo, FFAS score: -48.600), which also has a topsan page, and a yeast GNA1 (PDB code: 1i1d, FFAS score: -44.100). The superposition of 379751 and 1i1d indicates that N-terminal of 379751 fits well (green: 379751; 269 aa, cyan: 1i1d; 159aa). 


    The superposition of the CoA binding region in between 379751 and 1i1d is shown below. (green: 379751, cyan: 1i1d). 379751 contains CoA while 1i1d contains CoA and N-acetyl-D-glycosamine-6-phosphate. The conserved residues (Val75, Gly85 and Tyr115 of 379751,  and Val102, Gly112 and Tyr143 of 1i1d) are shown as stick.  Based on the sequence and structure similarity, 379751 is a putative acetyltransferase from the GNAT family.


    Ligand Summary

    - One CoA monomer is modeled based on the presence of clear and conclusive electron density.
    - EDO molecules from cryo condition were modeled.

    Reviews

    References

     

    No references found.

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