The Open Protein Structure Annotation Network
PDB Keyword


    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of putative alpha-N-acetylgalactosaminidase (NP_242736.1) from Bacillus halodurans at 2.00 A resolution. To be published
    Site JCSG
    PDB Id 3cc1 Target Id 376515
    Molecular Characteristics
    Source Bacillus halodurans c-125
    Alias Ids TPS1683,NP_242736.1, 104411 Molecular Weight 49502.70 Da.
    Residues 432 Isoelectric Point 5.35
    Sequence mevnrlsaltppmgwnswdcygasvteeevlgnaeymanhlkkygweyivvdiqwyeptanssaynpfa plcmdeygrllpatnrfpsakngagfkplsdaihdlglkfgihimrgiprqavyenspvlgstktarei ahtnsicpwntdmygvdptkegaqsyynslfelyaqwgvdfvkvddiaasrlydthleeikmiqraiqa cgrpmvlslspgpapikyahhfktnanmwritddfwddwsllyqmfercevwekhigtghwpdcdmlpl ghigirsvdgpggdrwtrftkdeqltmmnlwaicrsplmfggelrdndewtlslltnegilsinqksvl nrfvyreedkvawaangrngeayvalfnlhdqqktlqfrldmvgimetvqlfnvwdrsflqslapsesf qielkphqsmmlklspdr
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 2
    Resolution (Å) 2.00 Rfree 0.190
    Matthews' coefficent 3.30 Rfactor 0.162
    Waters 691 Solvent Content 62.75

    Ligand Information


    Google Scholar output for 3cc1
    1. 3DSIG 2010: The 6th Structural Bioinformatics and Computational Biophysics Meeting
    I An - 2010 - bcb.med.usherbrooke.ca

    Protein Summary

    Gene BH1870 from Bacillus halodurans encodes the NP_242736 protein with the N-terminal region (1-150) belonging to the melibiase (alpha-galactosidase) group (PF02065), which is  within clan CL0058.  BLAST sequence alignment also indicates that NP_242736 could be a member of the glycoside hydrolase family 31.

    Pre-SCOP classifies 3cc1 (N-terminal region) in the alpha/beta class, (trans)glycosidases superfamily, amylase catalytic domain family. The C-terminal region belongs to the all beta class, glycosyl hydrolase domain superfamily, alpha-amylase C-terminal beta-sheet domain family. DALI top hits are with alpha-galactosidases like 1uas, 3hg5, 1ktb (Z=35).

    3cc1 structure consists of three domains, the N-terminal 8 to 117 and 220 to 330, and the C-terminal 347 to 432. There are two subunits in each asymmetric unit as a dimer. The surface interacting calculation suggests the biomolecule of 3cc1 should be a tetramer instead of dimer.  Residue 311 mutated from Arg to His during protein expression.


    Ligand Summary

    SO4, PEG330, imidazole, glycerol





    No references found.

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