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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of glutathione S-transferase (NP_416804.1) from Escherichia coli K12 at 1.85 A resolution. To be published
    Site JCSG
    PDB Id 3bby Target Id 379351
    Molecular Characteristics
    Source Escherichia coli k12
    Alias Ids TPS1737,NP_416804.1, 86872 Molecular Weight 24324.40 Da.
    Residues 214 Isoelectric Point 5.27
    Sequence mskpaitlwsdahffspyvlsawvalqekglsfhiktidldsgehlqptwqgygqtrrvpllqiddfel sessaiaeyledrfapptweriypldlenrararqiqawlrsdlmpireerptdvvfagakkapltaeg kasaeklfamaehllvlgqpnlfgewciadtdlalminrlvlhgdevperlvdyatfqwqrasvqrfia lsakqsg
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 1.85 Rfree 0.195
    Matthews' coefficent 2.64 Rfactor 0.158
    Waters 230 Solvent Content 53.46

    Ligand Information


    Google Scholar output for 3bby

    Protein Summary

    The yfcF from Escherichia coli k12encodes glutathione S-transferase (subfamily 4).

    This structure is structural and sequentially homologous to Glutathione S-transferase (GST, eg 6gss) 

    The A71 (glu) is important by structural alignment, also A71 is a clearly defined Ramachandran outlier. It is interesting to note that 40-57 is disordered, this seems to correlate with empty active site, it is likely that this segment is important for substrate binding.

    PFAM entry http://pfam.sanger.ac.uk/family?acc=PF02798

    Putative catalytic tyrosine is Tyr18.  The additional analysis needs to be done to confirm Arg58 role in GSH binding.

    Ligand Summary





    No references found.

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