The Open Protein Structure Annotation Network
PDB Keyword


    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal Structure of Domain of Unknown Function with a Cystatin-Like Fold (ZP_00111510.1) from Nostoc punctiforme PCC 73102 at 1.80 A resolution. To be published
    Site JCSG
    PDB Id 2rgq Target Id 378141
    Molecular Characteristics
    Source Nostoc punctiforme pcc 73102
    Alias Ids TPS1711,NPUN_22DEC03_CONTIG1_REVISED_GENENPR3134, 3.10.450.50, 336080 Molecular Weight 16098.37 Da.
    Residues 143 Isoelectric Point 4.93
    Sequence meltaldkleimelaarfemsldkedvenylatfasdgalqgfwgiakgkeelrqgfyamldtfargkr hcssnaiiqgnydeatmesyltvvnredlnragsafvkdqvrkingkwylilrqievdpslpllqqsqq agana
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 3
    Resolution (Å) 1.80 Rfree 0.196
    Matthews' coefficent 2.34 Rfactor 0.163
    Waters 439 Solvent Content 47.38

    Ligand Information


    Google Scholar output for 2rgq
    1. Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard
    TC Terwilliger, PD Adams, RJ Read - Section D: Biological , 2009 - scripts.iucr.org
    2. Ligands in PSI structures
    A Kumar, HJ Chiu, HL Axelrod, A Morse - Section F: Structural , 2010 - scripts.iucr.org

    Protein Summary

    The Npun_R3134 gene from Nostoc punctiforme pcc 73102 encodes the YP_001866547 protein of unknown function that adopts a cystatin-like fold. HHPred strongly predicts homology of Npun_R3134 with the ring hydroxylating beta subunit of nonheme iron-containing dioxygenases (PF00866, P-value 6.8E-18 over residues 17-130), the association domain of protein kinases (PF08332, P-value 3E-15 over residues 7-123), scytalone dehydratases (PF02982, P-value 1.8E-11 over residues 2-129) and other members of the NTF2 clan all of which adopt the same (cystatin-like) fold.

     SCOP classifies 2rgq in the alpha+beta class, cystatin-like fold, NTF2-like superfamily, Rv3472-like family. DALI top hits are with the Rv3472 protein PDB:2chc (Z=19), the uncharacterized protein PDB:3b8l (Z=18), and the putative scyalone dehydratase PDB:3ef8 (Z=17).


    To do: look for presence of active site residues, iron/aromatic compound binding site; compare oligomerization states in the different members of the clan.

    Ligand Summary





    No references found.

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