The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of uncharacterized protein Tfu_2867 (YP_290923.1) from Thermobifida fusca YX at 1.95 A resolution. To be Published
    Site JCSG
    PDB Id 2qe6 Target Id 375255
    Molecular Characteristics
    Source Thermobifida fusca yx
    Alias Ids TPS1655,YP_290923.1, PF04672, 383064 Molecular Weight 29986.37 Da.
    Residues 273 Isoelectric Point 4.95
    Sequence msdeqhdsvwpppgldfskptiaraydallggkdnfeadraladyackhipglkesaienrkvlvrgvr flageagisqfldlgsglptvqnthevaqsvnpdarvvyvdidpmvlthgrallakdpntavftadvrd peyilnhpdvrrmidfsrpaaimlvgmlhylspdvvdrvvgayrdalapgsylfmtslvdtglpaqqkl aritrenlgegwartpeeierqfgdfelvepgvvytalwrpdepvdpdnlspgeqlgmagigrkka
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 2
    Resolution (Å) 1.95 Rfree 0.213
    Matthews' coefficent 2.60 Rfactor 0.171
    Waters 272 Solvent Content 52.64

    Ligand Information


    Google Scholar output for 2qe6
    1. Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard
    TC Terwilliger, PD Adams, RJ Read - Section D: Biological , 2009 - scripts.iucr.org
    2. Ligands in PSI structures
    A Kumar, HJ Chiu, HL Axelrod, A Morse - Section F: Structural , 2010 - scripts.iucr.org

    Protein Summary

    The Tfu_2867 gene (PF04672, also known as DUF574) from Thermobifida fusca YX encodes the YP_290923 protein of unknown function.

    2qe6 adopts a Rossman fold (2qe6-CATH). Pre-SCOP puts 2qe6 in the alpha/beta class, S-adenosyl-L-methionine-dependent methyltransferase superfamily. Dali top hits are with 3giw (Z-scr=36), and the carboxy-methyl transferases 2ob2 (Z-scr=19), and 1rjd (Z-scr=19). 2uyo is next with a Z-scr=18.

    Remote homolgy detection by HHpred indicates 2uyo, a methyltransferase, be a suitable target to infer function from. Structural comparison of these two proteins supports this hypothesis. The associated publication for 2uyo [Ref] suggests that this protein is a methyltransferase based on mandatory properties, still leaving space for uncertainities.See 3giw (identical to 3go4) for more details.

    Ligand Summary





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