The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of hypothetical protein (NP_244435.1) from Bacillus halodurans at 2.60 A resolution. To be published
    Site JCSG
    PDB Id 2o2z Target Id 372969
    Molecular Characteristics
    Source Bacillus halodurans c-125
    Alias Ids TPS1615,NP_244435.1, PF01933, 90263 Molecular Weight 34433.85 Da.
    Residues 322 Isoelectric Point 7.10
    Sequence mkkknvvvfgggtglsvllrglktfpvsitaivtvaddggssgrlrkeldipppgdvrnvlvalsevep lleqlfqhrfengnglsghslgnlllagmtsitgdfargisemskvlnvrgkvlpasnrsiilhgemed gtivtgessipkagkkikrvfltpkdtkplregleairkadvivigpgslytsvlpnllvpgiceaikq starkvyicnvmtqngetdgytasdhlqaimdhcgvgivddilvhgepisdtvkakyakekaepvivde hklkalgvgtisdyfvleqddvlrhnaskvseailegkprtsssiq
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 4
    Resolution (Å) 2.60 Rfree 0.224
    Matthews' coefficent 3.10 Rfactor 0.188
    Waters 280 Solvent Content 60.27

    Ligand Information


    Google Scholar output for 2o2z
    1. Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard
    TC Terwilliger, PD Adams, RJ Read - Section D: Biological , 2009 - scripts.iucr.org
    2. Ligands in PSI structures
    A Kumar, HJ Chiu, HL Axelrod, A Morse - Section F: Structural , 2010 - scripts.iucr.org
    3. Molecular insights into the biosynthesis of the F420 coenzyme
    F Forouhar, M Abashidze, H Xu, LL Grochowski - Journal of Biological , 2008 - ASBMB

    Protein Summary

    Gene BH3568 from Bacillus halodurans encodes the NP_244435 protein with sequence similarity to members of the UPF0052 (PF01933) and very remote similarity to the ubiquitin activating enzyme repeat 1-like group (cd01485). A Blast search classifies NP_244435 inside the CofD superfamily and hits with an e-val=2e-104 and 60% seq. id. the putative diguanylate cyclase ZP_02171651. Genome context analysis indicates a putative functional link with the UPF0042 protein BH3569 (score 0.97). The known protein located in similar genomic context is UvrC (UVRC_CACHD) (score 0.73).

    2o2z belongs to the SCOP alpha/beta class, CofD-like fold and (super)family. A DALI search provides significant hits with other UPF0052 structures like 2hzb, 2ppv, 2q7x, and 3cgw (Z=24; 20% seq.id.), a 2-phospho-L-lactate transferase ( [Ref] ). A molecule of NAD per chain is found in the 2o2z crystal structure interacting with residues T13, N217, M219, Y264, R300, H301.

    Ligand Summary





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