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2i6g

    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of putative METHYLTRANSFERASE (16420133) from SALMONELLA TYPHIMURIUM LT2 at 1.90 A resolution. To be Published
    Site JCSG
    PDB Id 2i6g Target Id
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    Molecular Characteristics
    Source
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    Alias Ids
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    TPS1424,16420133, 87269
    Molecular Weight
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    Da.
    Residues
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    Isoelectric Point
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    Sequence
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      BLAST   FFAS

    Structure Determination
    Method XRAY
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    Chains 1
    Resolution (Å)
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    Rfree
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    Matthews' coefficent 2.29 Rfactor 0.19
    Waters
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    Solvent Content 45.81

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    Ligand Information
    Ligands
    Metals

    Jmol

     
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    Google Scholar output for 2i6g
    1. New surface contacts formed upon reductive lysine methylation: Improving the probability of protein crystallization
    P Sledz, H Zheng, K Murzyn, M Chruszcz - Protein , 2010 - Wiley Online Library
     
    2. Structure and mechanism of the chalcogen-detoxifying protein TehB from Escherichia coli
    H Choudhury, A Cameron, S Iwata, K Beis - Biochem. J, 2011 - biochemj.org
     
    3. Crystallization and initial X-ray diffraction analysis of the tellurite-resistance S-adenosyl-L-methionine transferase protein TehB from Escherichia coli
    HG Choudhury, K Beis - Acta Crystallographica Section F: Structural , 2010 - scripts.iucr.org
     

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    Protein Summary

    The STM1608 gene from S. typhimurium codes for the NP_460567 amino acid sequence that is similar to the tellurite resistance protein TehB family (Pfam03848) that utilizes a methyltransferase activity in the detoxification of tellurite (Liu et al., 2000).

    2i6g belongs to the SCOP alpha/beta class, S-adenosyl-L-methionine-dependent methyltransferases superfamily, TehB-like family. DALI structural similarity search top hits are with the following PDB structures:
    PH0226 protein

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    (DALI Z-score 13.9; RMSD 2.8; 14% sequence identity for 146 superimposed residues);
    MJ0882 protein
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    (DALI Z-score 13.6; RMSD 3.1; 19% sequence identity for 147 superimposed residues);
    HI0319 protein
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    (DALI Z-score 13.4; RMSD 2.7; 14% sequence identity for 144 superimposed residues).

    Analysis of the crystallographic packing of 2i6g using the PQS server {Henrick, 1998 #73} indicates that a monomer is the biologically relevant form.

    Ligand Summary



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