The Open Protein Structure Annotation Network
PDB Keyword


    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of hypothetical protein (np_249484.1) from Pseudomonas aeruginosa at 1.95 A resolution. To be published
    Site JCSG
    PDB Id 2h9f Target Id 366300
    Molecular Characteristics
    Source Pseudomonas aeruginosa pao1
    Alias Ids TPS1476,NP_249484.1, PF04303 Molecular Weight 41707.04 Da.
    Residues 395 Isoelectric Point 5.44
    Sequence mahppqiripatylrggtskgvffrledlpescrvpgeardrlfmrvigspdpyaahidgmggatssts kcvilskssqpghdvdylygqvsidkpfvdwsgncgnlstgagafalhaglvdparipedgicevriwq anigktiiahvpvsggqvqetgdfeldgvtfpaaeivlefldpsddgedggaifptgnlvddlevpgvg tfkatminagiptvfvnaeeigyrgtelreeingdpqqlarferirvagalrmgliktpeeaatrqhtp kiafvapprdyrtasgklvaagdidllvralsmgklhhammgtaavaigtaaaipgtlvnlaagggers avrfghpsgtlrvgaeasqangewtvtkaimsrsarilmegwvrvpgdaf
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 1.95 Rfree 0.196
    Matthews' coefficent 4.18 Rfactor 0.168
    Waters 372 Solvent Content 70.36

    Ligand Information


    Google Scholar output for 2h9f
    1. Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard
    TC Terwilliger, PD Adams, RJ Read - Section D: Biological , 2009 - scripts.iucr.org
    2. Domain definition and target classification for CASP7
    ND Clarke, I Ezkurdia, J Kopp, RJ Read - Proteins: Structure, , 2007 - Wiley Online Library
    3. Detection of Functionally Important Regions in
    G Nimrod, M Schushan, DM Steinberg, N Ben-Tal - Structure, 2008 - Elsevier
    4. Ligands in PSI structures
    A Kumar, HJ Chiu, HL Axelrod, A Morse - Section F: Structural , 2010 - scripts.iucr.org
    5. The three_dimensional crystal structure of the PrpF protein of Shewanella oneidensis complexed with trans_aconitate: Insights into its biological function
    GS Garvey, CJ Rocco, JC Escalante_Semerena - Protein , 2007 - Wiley Online Library
    6. Crystal Structure and Putative Mechanism of 3-Methylitaconate-_-isomerase from Eubacterium barkeri
    M Velarde, S Macieira, M Hilberg, G Brker - Journal of molecular , 2009 - Elsevier
    7. Structural and functional studies of Secondary Metabolite AcylTransferase superfamily members from the trichothecene mycotoxin biosynthetic pathway
    GS Garvey - 2008 - books.google.com

    Protein Summary

    Gene PA0793 from Pseudomonas aeruginosa encodes the NP_249484 protein that belongs to the PrpF group (PF04303). Analysis of its genomic neighborhood indicates a possible functional link (score 0.9) with PA0794, a putative aconitate hydratase. SCOP classifies 2h9f structure in the alpha+beta class, diaminopimelate epimerase-like superfamily. DALI top hit is with the mehtylaconitate isomerase 2pwo and 2pvz (Z=47; 75% seq.id.).

    According to sequence (~80% id with YP_001187576) and structural (rmsd 2.4 Å over 256 residues with PDB id: 2pw0) similarity searches, NP_249484 protein is a methylaconitate isomerase [Ref].


    To do: check to see whether aconitate (bound to the enzyme in 2pw0 structure) can be docked onto 2h9f coordinates.

    Ligand Summary





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