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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of Peptide chain release factor 1 (RF-1) (SMU.1085) from Streptococcus mutans at 2.34 A resolution. To be published
    Site JCSG
    PDB Id 1zbt Target Id 358002
    Molecular Characteristics
    Source Streptococcus mutans ua159
    Alias Ids TPS1381,SMU.1085, 289705, 89876 Molecular Weight 40648.58 Da.
    Residues 359 Isoelectric Point 4.92
    Sequence mniydqlqavedryeelgellsdpdvvsdtkrfmelsreeansretvavyreykqvvqniadaqemikd asgdpeleemakeelknskvakeeyeeklrflllpkdpnddkniileirgaaggdeaalfagdllnmyq kyaenqgwkfevmeasangvgglkevvamvsgqsvysklkyesgahrvqrvpvtesqgrvhtstatvlv mpeveeveyeidpkdlrvdiyhasgaggqnvnkvatavriihlptnikvemqeertqqknrdkamkiir arvadhfaqiaqdeqdaerkstvgtgdrserirtynfpqnrvtdhrigltlqkldsilsgkldevidal ilydqtqkleelnk
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 2.34 Rfree 0.275
    Matthews' coefficent 3.18 Rfactor 0.221
    Waters 61 Solvent Content 61.03

    Ligand Information


    Google Scholar output for 1zbt
    1. The SAXS solution structure of RF1 differs from its crystal structure and is similar to its ribosome bound cryo-EM structure
    B Vestergaard, S Sanyal, M Roessle, L Mora - Molecular cell, 2005 - Elsevier
    2. Interactions of the release factor RF1 with the ribosome as revealed by cryo-EM
    U Rawat, H Gao, A Zavialov, R Gursky - Journal of molecular , 2006 - Elsevier
    3. Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard
    TC Terwilliger, PD Adams, RJ Read - Section D: Biological , 2009 - scripts.iucr.org
    4. On the combination of molecular replacement and single-wavelength anomalous diffraction phasing for automated structure determination
    S Panjikar, V Parthasarathy, VS Lamzin - Section D: Biological , 2009 - scripts.iucr.org
    5. Peptide release on the ribosome: mechanism and implications for translational control
    EM Youngman, ME McDonald, R Green - Annu. Rev. Microbiol., 2008 - annualreviews.org
    6. Release factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studies
    G Zoldk, L Redecke, DI Svergun - Nucleic acids , 2007 - Oxford Univ Press
    7. NMR structure of the peptidyl_tRNA hydrolase domain from Pseudomonas syringae expands the structural coverage of the hydrolysis domains of class 1 peptide
    KK Singarapu, R Xiao, T Acton, B Rost - Proteins: Structure, , 2008 - Wiley Online Library

    Protein Summary

    The gene SMU.1085 from Streptococcus mutans encodes a domain of peptide chain release factor 1 (RF-1) PF00472.  The protein belongs to the class of multi-domain alpha and beta (a+b) proteins and reveals release factor fold type SCOP75619.  RF1 is a protein that recognizes stop codons UAG and UAA, and promotes the release of a nascent polypeptide from tRNA on the ribosome.  Multiple structures of RF-1 from different organisms have been solved alone (1RQ0Thermotoga maritima) and in complex with ribosome (2B64Thermus thermophilus).

    Ligand Summary





    No references found.

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