The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of hypothetical protein (TM1380) from Thermotoga maritima at 2.12 A resolution. To be published
    Site JCSG
    PDB Id 1z85 Target Id 359772
    Molecular Characteristics
    Source Thermotoga maritima msb8
    Alias Ids TPS1433,TM1380, 452244, 429687 Molecular Weight 25096.84 Da.
    Residues 222 Isoelectric Point 8.63
    Sequence mphlfygtaqngevifdereahhmrvvrlkegdvieatdgngfsytcilkslkkktaaakivkveekek epteklsvvvpigrwertrfliekcvelgvdeiffhkfersqheisldkakivvreaakqckrylfpkv sfleklefsgnvitldldasqnlldanlegsitvvvgpeggfsekerellrssttivslgkkilrfeta ailtvgyialkkqki
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 2
    Resolution (Å) 2.12 Rfree 0.222
    Matthews' coefficent 2.83 Rfactor 0.171
    Waters 291 Solvent Content 56.14

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    Ligand Information


    Google Scholar output for 1z85
    1. The Buccaneer software for automated model building. 1. Tracing protein chains
    K Cowtan - Acta Crystallographica Section D: Biological , 2006 - scripts.iucr.org
    2. Structural and evolutionary bioinformatics of the SPOUT superfamily of methyltransferases
    KL Tkaczuk, S Dunin-Horkawicz, E Purta - BMC , 2007 - w09.biomedcentral.com
    3. Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family
    GN BASTUREA, KE RUDD, MP DEUTSCHER - Rna, 2006 - rnajournal.cshlp.org
    4. Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard
    TC Terwilliger, PD Adams, RJ Read - Section D: Biological , 2009 - scripts.iucr.org
    5. An iterative knowledge_based scoring function for proteinprotein recognition
    SY Huang, X Zou - Proteins: Structure, Function, and , 2008 - Wiley Online Library
    6. Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE
    GN Basturea, MP Deutscher - RNA, 2007 - rnajournal.cshlp.org
    7. Insights into the Catalytic Mechanism of 16S rRNA Methyltransferase RsmE (m 3 U1498) from Crystal and Solution Structures
    H Zhang, H Wan, ZQ Gao, Y Wei, WJ Wang - Journal of Molecular , 2012 - Elsevier

    Protein Summary

    The gene TM1380 from Thermotoga maritima encodes a putative 16S ribosomal RNA methyltransferase PF04452 COG1867 EC: (based on 92% sequence identity with the RsmE gene from Thermotoga petrophila).  The other name for this enzyme is tRNA (guanine-N(2)-)-methyltransferase.   The enzyme catalyzes the following reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N2-methylguanine. The genome vicinity of TM1380 includes the seryl-tRNA synthetase serS (TM1379).

    Pre-SCOP classfies 1z85 structure as follows: the 1-66 fragment inside the PUA domain-like superfamily, YggJ N-terminal domain-like family; the C-terminal 68-215 fragment in the alpha/beta knot superfamily, YggJ C-terminal domain-like family. DALI top hits are with the AQ165 protein PDB:2egv (Z=20), HI0303 protein PDB:1nxz (Z=20) and a putative methyltransferase PDB:3kw2(Z=20).

    Ligand Summary





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