The Open Protein Structure Annotation Network
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    Table of contents
    1. 1. Protein Summary
    2. 2. Ligand Summary
    3. 3. References

    Title Crystal structure of a putative NADPH-dependent oxidoreductase (GI: 18204011) from mouse at 2.10 A resolution. Proteins 56 629-633 2004
    Site JCSG
    PDB Id 1vj1 Target Id 354739
    Molecular Characteristics
    Source Mus musculus
    Alias Ids TPS1339,18204011, 282291, 289609, 289448, 289399, 281891, 89702 Molecular Weight 38052.47 Da.
    Residues 351 Isoelectric Point 5.38
    Sequence miiqrvvlnsrpgkngnpvaenfrveefslldalnegqvqvrtlylsvdpymrckmnedtgtdylapwq laqvadgggigiveeskhqklakgdfvtsfywpwqtkaildgnglekvdpqlvdghlsyflgaigmpgl tsligvqekghisagsnqtmvvsgaagacgslagqighllgcsrvvgicgtqekclfltselgfdaavn yktgnvaeqlreacpggvdvyfdnvggdisntvisqmnenshiilcgqisqynkdvpyppplppaveai rkernitrerftvlnykdkfepgilqlsqwfkegklkvketvakglenmgvafqsmmtggnvgkqivci sedssl
      BLAST   FFAS

    Structure Determination
    Method XRAY Chains 1
    Resolution (Å) 2.10 Rfree 0.216
    Matthews' coefficent 2.60 Rfactor 0.18
    Waters 153 Solvent Content 51.75

    Ligand Information


    Google Scholar output for 1vj1
    1. Exploring structurally conserved solvent sites in protein families
    CA Bottoms, TA White, JJ Tanner - : Structure, Function, and , 2006 - Wiley Online Library
    2. Predicting small ligand binding sites in proteins using backbone structure
    AJ Bordner - Bioinformatics, 2008 - Oxford Univ Press
    3. Mechanistic and structural studies of apoform, binary, and ternary complexes of the Arabidopsis alkenal double bond reductase At5g16970
    B Youn, SJ Kim, SGA Moinuddin, C Lee - Journal of Biological , 2006 - ASBMB
    4. Crystal structure of a putative NADPH_dependent oxidoreductase (GI: 18204011) from mouse at 2.10 resolution
    I Levin, R Schwarzenbacher - Proteins: Structure, , 2004 - Wiley Online Library
    5. Structure prediction of domain insertion proteins from structures of individual domains
    M Berrondo, M Ostermeier, JJ Gray - Structure, 2008 - Elsevier
    6. Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2
    YH Wu, TP Ko, RT Guo, SM Hu, LM Chuang - Structure, 2008 - Elsevier
    7. A fourier fingerprint-based method for protein surface representation
    MJ Bayley, EJ Gardiner, P Willett - Journal of chemical , 2005 - ACS Publications
    8. Analysis and Visualization of Long-Range Contacts and Networks in Homologous Families of Proteins
    KM Saravanan, S Selvaraj - Open Structural Biology Journal, 2009 - benthamscience.com
    9. Bioinformatics of protein bound water
    CA Bottoms - 2005 - mospace.umsystem.edu
    10. Predicting the structure and function of protein mutants
    M Berrondo - Dissertation Abstracts International, 2010 - graylab.jhu.edu

    Protein Summary

    Structure of putative NADPH-dependent oxidoreductase (GI:18204011; COG2130; Pfam00107) from Mouse comprises two distinct domains: the catalytic domain (residues 1-132; 311-351) and the nucleotide-binding domain (residues 133-310). Structure of the catalytic domain has GroES-like fold (SCOP sunid:50128) and  belongs to alcohol dehydrogenase-like family (SCOP sunid:50136). The nucleotide-binding domain has NAD(P)-binding Rossmann-fold domains (SCOP sunid:51734; 3 layers - a/b/a, where parallel beta-sheet of 6 strands has order 321456).

    Structural comparison with quinone oxidoreductase, shows that the large cleft between the two domains in GI:18204011 could easily accommodate an NADPH molecule and suggests a redox mechanism related to that of quinone oxidoreductase (PubMed:15229897).

    Ligand Summary





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